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[Biophysics seminar] Ajasja Ljubetič: “A completely de novo designed protein random walker/roller”

October 11 at 13:00 - 14:00

Powered protein walkers such as kinesin, dynein or myosin are responsible for most movements within the cell and for the transport of crucial cargo. Design of static monomeric and oligomeric protein structures has advanced tremendously; however large dynamic protein mechanisms have not yet been designed. I will present the design and characterization of a random protein walker that can diffuse along micro-meter long fibers. This represents a scaffold for future powered molecular robots.

The requirements for such a system are threefold: a track, attachment points and a walker/roller scaffold. I will briefly present reversible heterodimers that I developed to serve as attachment points. These heterodimers behave well as monomers, have a range of affinities and fast binding/exchange kinetics in solution.

Next, I will show how I rigidly fused heterodimers and designed helical repeat (DHR) proteins to de-novo designed fibers to form a track for the walker. This turned out to be the hardest part of the project.

Finally, I will outline the different walker/roller scaffolds I used and show trajectories obtained from single molecule microscopy experiments.

The meeting will take place in the seminar room of physics (106).


October 11
13:00 - 14:00


Seminarska soba fizike (106)
Jozef Stefan Institute (Jamova 39)